Effect of surface sterilizing agents on in vitro culture establishment of guava (Psidium guajava L.).

P.R. Meghwal; H.C. Sharma; S.K. Singh

ISSN: 0972-1045

Effect of surface sterilizing agents on in vitro culture establishment of guava (Psidium guajava L.).

Journal of Applied Horticulture, 2000, 2(2): 94-95.

Effect of surface sterilizing agents on in vitro culture establishment of guava (Psidium guajava L.).

P.R. Meghwal, H.C. Sharma and S.K. Singh

Division of Fruits and Horticultural Technology, IARI, New Delhi - 110 012, India.

DOI: https://doi.org/10.37855/jah.2000.v02i02.28

Key words: browning, disinfectants, guavas, hydrogen peroxide, in vitro culture, in vitro regeneration, mercuric chloride, micropropagation, phenolic compounds, shoots, silver nitrate, tissue culture

Abstract:

A method was standardized for the quick establishment of aseptic cultures in guava from mature field-grown stock plants for micropropagation through enhanced axillary branching technique. The maximum number of aseptic explants with shoot proliferation was obtained by a combination of surface sterilizing agents involving hydrogen peroxide (10%), silver nitrate (0.25%), and mercuric chloride (0.05%) treatment of explants one by one for five, six, and three minutes, respectively. The problem of phenolic browning was also minimized to a great extent by leaching of phenolic compounds due to agitation in antioxidant solution as well as by proper drying of explant prior to inoculation.